Immune colloidal gold marked technology based on nano colloidal gold (often referred to as "colloidal gold) as tracer marker application in the antigen antibody detection of a new type of immune markers, introduce the colloidal gold by Faulk equals 1971 immunochemical established a method of immunology Leuvering as early as in 1980 reported using colloidal gold detection of human chorionic gonadotropin (m CG) agglutination test. At present, the technology has been rapidly applied and popularized in the fields of medical clinical inspection, food safety inspection and animal epidemic surveillance. Colloidal gold immunofiltration and colloidal gold immunochromatography are the most widely studied and applied techniques in the veterinary field.
Colloidal gold is negatively charged in a weak base environment and can form a firm electrostatic bond with positively charged groups of protein molecules. This binding does not affect the biological properties of the protein. Colloidal gold particles have A strong adsorption function to proteins due to their high surface charge. Colloidal gold particles can be noncovalently combined with staphylococcus A protein (SPA), immunoglobulin, toxin, glycoprotein, enzyme, antibiotic, hormone, and bovine serum albumin peptide conjugate to form colloidal gold proteins. With the gold particles with high electron density as the marker, the visible pink spots may appear when the gold particles aggregate to a certain density at the antigen body reaction site on the solid phase carrier. Therefore, colloidal gold, as a marker, can be used in immunoelectron microscopy, conventional light microscopy, quantitative and qualitative studies of antigens, as well as qualitative or semi-quantitative rapid detection of antigens or antibodies in vitro immunochromatography.
Immunocolloidal gold technology has good stability, easy preservation and transportation. The experimental results can be preserved for a long time. The operation is simple and fast, and the results are easy to be observed and judged. The operation process is just immersing the strip into the sample. The colloidal gold is used as an indicator in the reaction process to form the color mark after the immunoanalysis process. The whole detection process only takes 3-10 minutes; It is not toxic to the operator. In colloidal gold immunodetection, there are no harmful substances such as radioisotopes, o-phenylenediamine that pollute the environment and cause damage to the operator. Gold and silver do not harm to the body, no pollution to the environment; In addition to the commercial reagent does not need any instrument equipment, save the cost of the instrument, but also simplify the operation process, more suitable for field application.
TRUNNANO (aka. Luoyang Tongrun Nano Technology Co. Ltd.) is a trusted global chemical material supplier & manufacturer with over 12 years experience in providing super high quality chemicals and Nano materials. The Colloidal Gold produced by our company has high quality. Lower, please contact us if necessary.
