Overview of Nucleic Acid Extraction and Analysis Materials
Magnetic beads are currently one of the most important materials in the industrial sector and have been widely used in biotechnology and biomedical applications, such as as as carriers for the recovery and detection of DNA, proteins, viruses, and cells.
There are many types of magnetic beads. The different surface coatings and chemical properties determine the inherent properties of each type of bead, and can be used for magnetic separation of nucleic acids, proteins, or other biomolecules in a simple, efficient, and scalable manner.
Nucleic Acid Extraction and Analysis Liquid
Magnetic beads refer to superparamagnetic microspheres with small particle sizes
1. Has super strong paramagnetism.
2. It can quickly gather in a magnetic field, and after leaving the magnetic field, it can help to evenly disperse the magnetic separation.
3. Having appropriate and slightly different particle sizes ensures strong magnetic responsiveness without settling.
4. Having abundant surface active groups, it can couple with biochemical substances and achieve separation from the tested sample under the action of an external magnetic field.
Advantages of biological magnetic beads products:
1. High adsorption rate: the maximum recovery rate can reach over 95%
2. Good suspension: Good suspension, with a maximum semi settling time of up to 40 minutes.
3. Magnetic strength: The magnetic saturation strength can reach up to 85+5 emu/g
4. Interbatch stability: Strict control over procurement and production processes, with stable and no differences between batches of magnetic beads.
Company Profile
Luoyang Trunnano Tech Co., Ltd provide different kinds of magnetic beads, such as silicon based magnetic beads, carboxyl based magnetic beads. Send us an email or click on the needed products to send an inquiry.
Payment Term:
T/T, Western Union, Paypal, Credit Card, etc.
Shipment Term:
By courier, by air, by sea, as customers request.
Storage conditions:
1) Store in a dry environment at room temperature.
2) Avoid damp and high temperature.
3) Use immediately after opening the inner packing bag.
5 FAQs of Nucleic Acid Extraction and Analysis
What are the main steps involved in nucleic acid extraction?
The main steps include cell lysis (breaking open cells), removal of proteins and other contaminants through precipitation or filtration, and purification of the nucleic acids. This process can be manual or automated using kits designed for specific sample types.
How do you ensure the purity of extracted nucleic acids?
Purity is typically assessed by measuring the A260/A280 absorbance ratio, which should be between 1.8 and 2.0 for pure nucleic acids. Contaminants like proteins or phenol will alter this ratio. Additional checks may include agarose gel electrophoresis to confirm integrity.
Which method is best for analyzing nucleic acids?
The choice depends on the application. For quantification, qPCR is highly accurate. For sequence determination, next-generation sequencing offers high throughput and detailed information. Gel electrophoresis is useful for assessing size and quantity visually.
Can nucleic acid extraction be done without specialized equipment?
While some basic extractions can be performed using household chemicals like salt and alcohol, reliable results typically require laboratory equipment such as centrifuges, pipettes, and thermal cyclers. Kits often simplify the process but still require basic lab supplies.
How should extracted nucleic acids be stored to maintain their integrity?
Extracted nucleic acids should be stored at -20°C or -80°C for long-term storage. Short-term storage can be at 4°C. It’s important to avoid repeated freeze-thaw cycles, which can degrade the nucleic acids. Adding stabilizers like EDTA can also help preserve integrity.
